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检查荧光模式Cryosectioning酵母社区
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Biologia
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JoVE Journal Biologia
Cryosectioning Yeast Communities for Examining Fluorescence Patterns
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检查荧光模式Cryosectioning酵母社区

DOI:
10.3791/50101-v

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07:03 min

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December 26, 2012

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1Division of Basic Sciences,Fred Hutchinson Cancer Research Center

Capitoli

  • 00:05Titolo
  • 00:58Fixing Yeast Communities
  • 03:40Sectioning Frozen Communities for Fluorescence Imaging
  • 05:15Results: Fluorescence Images of Vertical Cross-sections of Yeast Communities
  • 06:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了一个协议冻结和cryosectioning的酵母菌社区观察荧光细胞的内部模式。该方法依赖于甲醇固定，OCT-嵌入社区内的荧光蛋白失活的细胞没有保留的空间分布。

Tags

Keywords: CryosectioningYeast CommunitiesFluorescence PatternsOptical SectioningConfocal MicroscopyTwo-photon MicroscopySpatial OrganizationSaccharomyces CerevisiaeMethanol FixationOCT CompoundCryostatFluorescent ProteinsGene ExpressionMicrobial Communities

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