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Neuroscience

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JoVE Journal Neuroscience
Ex utero Electroporation and Whole Hemisphere Explants
 

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Article DOI: 10.3791/50271-v • 13:47 min • April 3rd, 2013
April 3rd, 2013 •
*1, *1, *1, 1
1Department of Neuroscience and Physiology, SUNY Upstate Medical University
* These authors contributed equally

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This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.

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Ex Utero Electroporation Whole Hemisphere Explants Cortical Development Neuronal Differentiation Organotypic Environment Cortical Slice Explants Cellular Lamination Migration Patterns Preplate Splitting Cortical Layer Six Ex Utero Electroporation (EUEP) GFP Expression Dorsal Medial Cortex Pharmacological Intervention Live Imaging Approaches
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