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Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
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Neuroscienze
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JoVE Journal Neuroscienze
Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

DOI:
10.3791/57842-v

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07:16 min

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August 16, 2018

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, ,
1Discovery Neuroscience, Pharma Research and Early Development,Roche Innovation Center Basel, 2HiQScreen Sàrl 6

Capitoli

  • 00:04Titolo
  • 00:54Receptor Expression and Recordings in Xenopus Oocytes
  • 02:16Electrophysiological Recordings in Rat Brain Slices
  • 05:39Results: A GABAA NAM Reduces Paired-pulse Inhibition in Hippocampal Slices
  • 06:49Conclusion

Summary

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Here, we present protocols to discover compounds active at GABAA receptors, from the binding to the physiology and pharmacology.

Tags

GABA-A ReceptorRadioligand BindingElectrophysiologyXenopus OocytesRodent Brain SlicesCompound ScreeningSubtype SelectivityEfficacyMicroinjectionVoltage ClampPopulation Spike RecordingHippocampus

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