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Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

DOI:
10.3791/50329-v

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11:18 min

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April 02, 2013

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, ,
1Department of Pharmacology,University of Illinois at Chicago , 2Department of Anesthesiology,University of Illinois at Chicago

Capitoli

  • 00:05Titolo
  • 01:20Intravital Microscope and Cremaster Muscle Preparation for Intravital Microscropy
  • 02:30Intravital Microscopy for TNFα-induced Vascular Inflammation
  • 03:33Data Analysis for the Venular Inflammation Model
  • 06:06Intravital Microscopy for Laser-induced Arteriolar Thrombosis
  • 06:58Data Analysis for the Arteriolar Thrombosis Model
  • 08:20Results: Representative Heterotypic Interactions of Platelets and Neutrophils during TNFα-induced Vascular Inflammation and Platelet Thrombus at the Site of Laser-induced Arteriolar Injury in Live Mice
  • 10:52Conclusion

Summary

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Here we report an experimental technique of fluorescence intravital microscopy to visualize heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice. This microscopic technology will be valuable to study the molecular mechanism of vascular disease and to test pharmacologic agents under pathophysiological conditions.

Tags

Platelet-neutrophil InteractionsVascular InflammationThrombus FormationEndotheliumIntravital MicroscopyTNF-αLaser-induced InjuryCremaster Muscle

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