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Neuroscience

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JoVE Journal Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
 

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Article DOI: 10.3791/50400-v • 11:46 min • May 16th, 2013
May 16th, 2013 •
1, 2, 3
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney, 2The MARCS Institute, University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney

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This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

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Dynamic Clamp Synaptic Conductances Artificial Conductances Mouse Retinal Ganglion Cells Patch Clamp Techniques Voltage Clamp Configuration Current Clamp Configuration Excitatory Inputs Inhibitory Inputs Neuronal Excitability Modified Current Clamp Technique Conductance Clamp Injection Of Current Real-time Feedback Membrane Potential Experimental Procedures Whole-cell Configuration Conductance Waveforms
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