Rivista
/
Neuroscienze
/
胰蛋白酶精华协议分析视网膜血管小鼠模型
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
Please note that all translations are automatically generated. Click here for the English version.

胰蛋白酶精华协议分析视网膜血管小鼠模型

DOI:
10.3791/50489-v

•

09:03 min

•

June 13, 2013

•

, ,
1Department of Ophthalmology,Northwestern University Feinberg School of Medicine

Capitoli

  • 00:05Titolo
  • 01:34Retinal Preparation
  • 02:22Water Washes and Trypsin Digest
  • 03:33Separating the Vasculature
  • 05:32Flat-mounting the Vasculature
  • 06:13Results: Representative Images of Trypsin Digest
  • 08:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

胰蛋白酶的消化是一个最常用的方法来分析视网膜血管。这份手稿中详细介绍的方法，包括关键的改动，优化技术，并删除无血管组织，同时保持整体架构的船只。

Tags

Retinal VasculatureTrypsin DigestMouse ModelFlat-mount Retinal Blood VesselsRetinal Vascular ChangesMicroaneurysmsCapillary DegenerationEndothelial To Pericyte RatiosMouse EyeRetinal Blood VesselsRetinal Blood Vessel Architecture

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

从一个暗适应切片制备小鼠视网膜神经细胞的膜片钳录音

解剖鼠标眼的视网膜色素上皮的全山

一个视神经挤压伤的小鼠模型，研究视网膜神经节细胞生存

视网膜节的形态分析

从大鼠脑的解剖脑表面血管及相关脑膜和脉络丛的可视描述，

在小鼠视网膜神经细胞，实现动态夹具与突触和人工电导

角膜微囊含量：血管生成在小鼠眼模型

视频成像和时空地图来分析胃肠动力小鼠

协议隔离威利斯的鼠标圈

小鼠视网膜冷冻节的制备,用于免疫性植物化学

Read Article