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Immunology and Infection

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JoVE Journal Immunology and Infection
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
 

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Article DOI: 10.3791/50491-v • 09:39 min • May 30th, 2013
May 30th, 2013 •
1, 2, 3
1Department of Biological Sciences, Kingsborough Community College, 2Institute for Cellular and Molecular Biology, University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics, Kean University

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Riepilogo
Automatic Translation

We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.

Tags

Fatty Acids Cholera Toxin Human Intestinal Epithelial Cells BALB/c Mouse Macrophages Cell Proliferation Assays Mucosal Immunity Cholera Prevention
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