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实时成像检测方法的评估钙的作用<sup> 2 +</sup>和鞘磷脂酶在成孔毒素伤口的修复
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Biologia
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JoVE Journal Biologia
Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
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实时成像检测方法的评估钙的作用<sup> 2 +</sup>和鞘磷脂酶在成孔毒素伤口的修复

DOI:
10.3791/50531-v

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18:25 min

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August 25, 2013

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, ,
1Department of Cell Biology and Molecular Genetics,University of Maryland

Capitoli

  • 00:05Titolo
  • 01:41Transcriptional Silencing of ASM
  • 04:18Preparation of Reagents for Live Microscopy
  • 05:29Live Cell Imaging of FM1-43 Influx into SLO Treated and Untreated Cells
  • 10:21Quantification of FM1-43 Influx into Cells
  • 14:03Results: Ca2+ and Sphingomyelinase are Involved in Plasma Membrane Repair
  • 17:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

细胞暴露于所述亲脂性染料FM1-43的实时成像可以精确测定由该成孔毒素从质膜除去动力学。这是一个敏感的测定法可以用于评估要求的Ca 2 +，鞘磷脂和其它因素对质膜修复。

Tags

Live ImagingCa2+SphingomyelinasePore-forming ToxinPlasma Membrane RepairFM1-43Streptolysin OExocytosisEndocytosis

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