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1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry,Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST,Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST,Center for Nanotechnology Innovation
Capitoli
- 00:05Titolo
- 02:30Synthesis of the Sensors
- 04:20In vitro pH Measurements
- 05:36Cell Culture and Electroporation
- 06:38pH Sensing in Living HeLa Cells
- 08:47In vivo Sample Preparation
- 10:30pH Imaging in Mouse Brain
- 12:24Representative Analysis of Dendrimer-based pH Sensor Fluorescence
- 15:45Conclusion
Fluoreszenz-Sensoren sind mächtige Werkzeuge in Life Science. Hier beschreiben wir eine Methode zu synthetisieren und zu verwenden Dendrimerbasis Fluoreszenzsensoren auf pH in lebenden Zellen und in vivo zu messen. Die dendritische Gerüst verbessert die Eigenschaften von konjugierten Fluoreszenzfarbstoffe, die zu verbesserten Sensoreigenschaften.
