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提高蛋白质结晶的成功率随机Microseed矩阵筛选
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Biologia
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JoVE Journal Biologia
Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
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提高蛋白质结晶的成功率随机Microseed矩阵筛选

DOI:
10.3791/50548-v

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12:24 min

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August 31, 2013

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, , , , , ,
1School of Biochemistry,University of Bristol, 2Douglas Instruments

Capitoli

  • 00:05Titolo
  • 01:58Preparation of the Seed Stock
  • 06:09Establishment of Crystallization Trays
  • 08:45Inspection of Crystallization Trays
  • 09:40Representative rMMS Experiment
  • 11:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了随机microseed矩阵筛选的一般方法。这种技术被示为显著增加的蛋白质结晶筛选实验的成功率，减少需要优化，并提供晶体的数据收集和配体浸泡实验的可靠供应。

Tags

Keywords: Protein CrystallizationRandom Microseed Matrix ScreeningRMMSVapor DiffusionSitting DropHanging Drop96-well24-wellLiquid Handling RoboticsProtein Phase DiagramMetastable ZoneCrystal QualityCrystal SupplySoaking Experiments

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