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Parabiosis in Mice: A Detailed Protocol
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JoVE Journal Medicina
Parabiosis in Mice: A Detailed Protocol

Parabiosis in Mice: A Detailed Protocol

DOI:
10.3791/50556-v

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07:56 min

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October 06, 2013

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, , , , ,
1Department of Medicine-Division of Cardiology,University of California, Los Angeles, 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, 3Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:06Surgical Preparations
  • 02:22Parabiosis Surgery
  • 04:35Post-operative Recovery
  • 05:23Confirmation and Reverse Procedure
  • 07:05Anticipated Outcome Using GFP
  • 07:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

Parabiotic joining of two organisms leads to the development of a shared circulatory system. In this protocol, we describe the surgical steps to form a parabiotic connection between a wild-type mouse and a constitutive GFP-expressing mouse.

Tags

ParabiosisMiceSurgical UnionBlood CirculationMicrovasculatureImmune ReactionGFP Expressing MouseWild Type MouseBlood ChimerismFlow Cytometry

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