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Bioengineering

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JoVE Journal Bioengineering
Self-reporting Scaffolds for 3-Dimensional Cell Culture
 

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Article DOI: 10.3791/50608-v • 14:49 min • November 7th, 2013
November 7th, 2013 •
1, 2, 3, 1
1School of Molecular Medical Sciences, University of Nottingham, 2Division of Drug Delivery and Tissue Engineering, University of Nottingham, 3Laboratory of Biophysics and Surface Analysis, University of Nottingham

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Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.

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Self-reporting Scaffolds 3-dimensional Cell Culture In Vivo Microenvironment Cell-cell Interactions 3D Cellular Construct Molecular Events Analyte Concentration Gradients Biocompatible PH Responsive Sol-gel Nanosensors Poly(lactic-co-glycolic Acid) (PLGA) Electrospun Scaffolds Mammalian Cells Microenvironmental PH Intracellular Environment Intracellular PH (pHi) Monitoring
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