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Immunology and Infection

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JoVE Journal Immunology and Infection
Forward Genetic Approaches in Chlamydia trachomatis
 

Forward Genetic Approaches in Chlamydia trachomatis

Article DOI: 10.3791/50636-v • 09:03 min • October 23rd, 2013
October 23rd, 2013 •
1, 1
1Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis, Duke University Medical Center

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We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.

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Genetic Analysis Chlamydia Trachomatis Forward Genetics Chemical Mutagenesis Whole-genome Sequencing Genotype-phenotype Association Recombinant Strains Genetic Tools Intractability Sexually Transmitted Diseases Ocular Infections
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