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Uma<em> In vitro</em> Modelo para estudar respostas imunes de sangue periférico humano células mononucleares para Vírus Respiratório Sincicial Humano Infecção
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
DOI:

09:01 min

December 10, 2013

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Capitoli

  • 00:05Titolo
  • 01:12Human Respiratory Syncytial Virus (RSV) A2 Culture
  • 02:39RSV Purification
  • 04:14Peripheral Blood Mononuclear Cell (PBMC) Isolation from Healthy Volunteer Blood
  • 05:50Stimulation of PBMC with Live RSV
  • 06:30Results: Representative Human PBMC Immune Responses to RSV Infection
  • 08:26Conclusion

Summary

Traduzione automatica

Human respiratory syncytial virus (HRSV) can cause severe bronchiolitis in young infants. Part of the pathogenesis of severe HRSV disease is caused by the host immune response. Stimulation of primary human immune cells with HRSV provides a fast and reproducible model system to study activation of inflammatory pathways and infection.

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