Rivista
/
/
Redding van recombinant Newcastle Disease Virus uit cDNA
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Rescue of Recombinant Newcastle Disease Virus from cDNA
DOI:

10:55 min

October 11, 2013

, ,

Capitoli

  • 00:05Titolo
  • 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
  • 02:40Transfection of Mammalian Cells
  • 03:48Co-culture of Mammalian Cells with Avian Cells
  • 05:22Infection of Chicken Embryonated Eggs
  • 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
  • 07:52Hemagglutination (HA) Assay
  • 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
  • 10:33Conclusion

Summary

Traduzione automatica

Newcastle Disease Virus (NDV) is uitgebreid bestudeerd in de afgelopen jaren om nieuwe vectoren te ontwikkelen voor vaccinatie en therapie, onder anderen. Deze studies zijn mogelijk gemaakt door technieken om recombinant virus te redden van cDNA, zoals hier beschreven.

Video correlati

Read Article