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成像变性的胶原股<em>在体内</em>和<em>体外</em>通过光引发杂交笼胶原蛋白仿生肽
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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成像变性的胶原股<em>在体内</em>和<em>体外</em>通过光引发杂交笼胶原蛋白仿生肽

DOI:
10.3791/51052-v

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07:03 min

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January 31, 2014

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, , ,
1Department of Bioengineering,University of Utah, 2Department of Radiology and Radiological Science,Johns Hopkins University School of Medicine, 3Institute for NanoBiotechnology,Johns Hopkins University

Capitoli

  • 00:05Titolo
  • 01:25Photo-activation and IV Injection of Caged Collagen Mimetic Peptides (CMPs)
  • 02:22Near Infrared (NIR) Fluorescence Imaging of Skeletal Collagen Remodeling Activity In vivo
  • 03:31Visualization of Collagens in Ex vivo Tissue Sections
  • 05:19Representative Images
  • 06:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

本程序演示了在附近的胶原重塑活动在小鼠红外荧光成像以及胶原蛋白的使用笼胶原蛋白模拟肽，可以是光引发的杂交与变性的胶原链组织切片的体外活体染色。

Tags

CollagenDenatured CollagenExtracellular MatrixCaged Collagen Mimetic PeptidePhoto-triggered HybridizationIn Vivo ImagingEx Vivo ImagingProteasesTriple Helix

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