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Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis
 

Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis

Article DOI: 10.3791/51073-v • 10:55 min • March 16th, 2014
March 16th, 2014 •
1, 1
1Department of Organismic and Evolutionary Biology, Harvard University

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Automatic Translation

The amphipod Parhyale hawaiensis is a promising model organism for studies of crustacean embryology and comparative arthropod development and evolution. This protocol describes a method for manual removal of single blastomeres from early cleavage stage embryos of Parhyale.

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Keywords: Parhyale Hawaiensis Amphipod Crustacean Eight-cell Embryo Blastomere Ablation Germ Layer Fate Manual Ablation Holoblastic Cleavage Model Organism Development Cell Removal
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