Rivista
/
Biologia
/
Nem Måling af diffusionskoefficienter af EGFP-tagget plasmamembranproteiner Brug k-Space Billede korrelationsspektroskopi
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

Nem Måling af diffusionskoefficienter af EGFP-tagget plasmamembranproteiner Brug k-Space Billede korrelationsspektroskopi

DOI:
10.3791/51074-v

•

11:43 min

•

May 10, 2014

•

, , , ,
1Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center,Aarhus University, 2Departments of Chemistry and Physics,McGill University

Capitoli

  • 00:05Titolo
  • 01:15Import the Image Sequence of Interest into an Imaging Analysis Program
  • 02:37Perform the Analysis
  • 06:52Calculate Average of the Diffusion Coefficient
  • 07:56Results: Example ROI Crops, Diffusion, and k2 Plots
  • 11:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

Dette dokument indeholder en trin for trin guide til udsving analyse teknik k-Space Billede korrelationsspektroskopi (VIF) til måling diffusionskoefficienter af fluorescens-mærkede plasmamembranproteiner i levende pattedyrceller.

Tags

Diffusion CoefficientsEGFPPlasma Membrane ProteinsK-Space Image Correlation Spectroscopy (kICS)Fluorescence MicroscopyImage AnalysisAquaporin-3Membrane Protein Diffusion

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

Crystallization of Membrane Proteins in Lipidic Mesophases

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Kortlægning af molekylær diffusion i plasmamembranen ved Multiple-Target Tracing (MTT)

Anvendelse af en robot til Højkapacitetsforskning Krystallisation af membranproteiner i Lipidiske mesofaser

The Cell-baserede L-Glutathion Protection Analyser til Undersøgelse Endocytose og genanvendelse plasmamembranproteiner

Imaging plasmamembranen Deformations Med pTIRFM

Brug Digital Image Korrelation til Karakterisere Lokale Stammer på Vaskulære vævsprøver

Read Article