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最快在西镇：对经典蛋白质印迹分析现代风味
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Biologia
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JoVE Journal Biologia
The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
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最快在西镇：对经典蛋白质印迹分析现代风味

DOI:
10.3791/51149-v

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11:43 min

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February 05, 2014

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,
1Diller Cancer Research Building,University of California, San Francisco

Capitoli

  • 00:05Titolo
  • 01:48Preparation of Whole Cell Lysates from Cell Culture
  • 04:00Sample Preparation and Electrophoresis
  • 05:50Protein Transfer Using the iBlot Dry Blotting System
  • 07:08Infrared Fluorescent Protein Detection
  • 09:14Results: Improved Western Blot Sensitivity, Quality, and Accuracy
  • 11:13Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议探索的最新进展进行免疫印迹分析。这些新颖的改进采用的Bis-Tris凝胶系统用35分钟电泳运行时，一个7分钟的干印迹转移系统和红外荧光蛋白的检测和成像，其产生更高的分辨率，质量，灵敏度和西数据的改进的准确度。

Tags

Keywords: Western BlotBis-Tris GelIBlot Dry BlottingInfrared Fluorescent ImagingProtein DetectionQuantificationLinearityDynamic RangeEnhanced ResolutionFaster Protocol

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