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定量检测抗体诱导补体激活的方法对红血细胞
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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定量检测抗体诱导补体激活的方法对红血细胞

DOI:
10.3791/51161-v

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06:29 min

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January 29, 2014

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, ,
1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Capitoli

  • 00:05Titolo
  • 01:21Complement Deposition Analysis and Quantification of Hemolysis
  • 04:34Results: Quantitative Detection of Antibody-induced Complement Activation on RBCs
  • 06:02Conclusion

Summary

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在这里，我们描述了两个测定法用于测定补体活化诱导的抗红细胞。过电流测定法的主要优点是它们的数量和易于理解的性质。

Tags

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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