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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

DOI:
10.3791/51161-v

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06:29 min

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January 29, 2014

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, ,
1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Chapters

  • 00:05Title
  • 01:21Complement Deposition Analysis and Quantification of Hemolysis
  • 04:34Results: Quantitative Detection of Antibody-induced Complement Activation on RBCs
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe two assays for measuring complement activation induced by antibodies against red blood cells. The major advantage over the current assays is their quantitative and easy-to-interpret nature.

Tags

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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