JoVE Journal
Immunology and Infection
Immunology and Infection
This Contenuto is Open Access.
Capitoli
Riepilogo
Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).