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Journal / Biology / Efficient Production and Purification of…
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Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
 

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Article DOI: 10.3791/51206-v • 13:52 min • March 19th, 2014
March 19th, 2014 •
1, 1
1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford

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Kindlins are fundamental to cell adhesion through integrins but studies of them have been hampered by the difficulty encountered in expressing them recombinantly in bacterial hosts. We describe here methods for their efficient production in baculovirus-infected insect cells.

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Keywords: Recombinant Murine Kindlin-3 Insect Cells Baculovirus Sf9 Cells Protein Purification Biophysical Studies Integrins Cell Signaling Platelet Aggregation Leukocyte Rolling Osteoclast Formation
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