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Bioengineering

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JoVE Journal Bioengineering
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
 

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Article DOI: 10.3791/51215-v • 10:13 min • June 3rd, 2014
June 3rd, 2014 •
*1,2,3, *1,2,3, 1, 1, 1,4, 1,2,3
1The BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Burns Hospital, 4Harvard University School of Engineering and Applied Sciences
* These authors contributed equally

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This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

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Keywords: Microfluidic Platform Neutrophil Chemotaxis Unprocessed Whole Blood Neutrophil Migration Blood Droplet Chemoattractant Red Blood Cell Filtration Finger Prick Venous Blood Purified Neutrophils
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