Rivista
/
Bioingegneria
/
Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

DOI:
10.3791/51302-v

•

11:11 min

•

June 10, 2014

•

, , ,
1Department of Physics,University of Massachusetts Boston

Capitoli

  • 00:05Titolo
  • 01:25Culturing Tumor Spheroids
  • 02:54Preparing 3D Tumor Spheroids Embedded in ECM-containing Tracer Probes
  • 05:33Construction of Sample Point Grid and Video Acquisition at Each Point
  • 07:34Video Data Analysis for Computation of Rheological Properties at Each Spatial Position
  • 09:10Results: Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models
  • 10:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

Particle-tracking microrheology can be used to non-destructively quantify and spatially map changes in extracellular matrix mechanical properties in 3D tumor models.

Tags

3D Tumor ModelExtracellular MatrixParticle-tracking MicrorheologyViscoelastic Shear ModulusMechanosensitive InteractionsMatrix RemodelingPancreatic Cancer

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/51302?article_type=v

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
check_url/it/51302?article_type=v

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology
check_url/it/51302?article_type=v

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
check_url/it/51302?article_type=v

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
check_url/it/51302?article_type=v

A Protocol for Real-time 3D Single Particle Tracking
check_url/it/51302?article_type=v

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
check_url/it/51302?article_type=v

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
check_url/it/51302?article_type=v

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
check_url/it/51302?article_type=v

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
check_url/it/51302?article_type=v

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Read Article