Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Lineage-reprogramming of Pericyte-derived Cells…
JoVE Journal
Neuroscience

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Neuroscience
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
 

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Article DOI: 10.3791/51433-v • 09:36 min • May 12th, 2014
May 12th, 2014 •
1, 2, 3, 1,4
1Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

Capitoli

Riepilogo
Automatic Translation

Targeting brain-resident cells for direct lineage-reprogramming offers new perspectives for brain repair. Here we describe a protocol of how to prepare cultures enriched for brain-resident pericytes from the adult human cerebral cortex and convert these into induced neurons by retrovirus-mediated expression of the transcription factors Sox2 and Ascl1.

Tags

Keywords: Lineage-reprogramming Pericyte Adult Human Brain Induced Neurons Direct Conversion Neurogenesis In Vitro Modeling Brain Repair Cerebral Cortex PDGF Receptor-β CD146 Sox2 Ascl1 Pericyte-derived Induced Neurons Patch-clamp Recording
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter