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Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy
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JoVE Journal Medicina
Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

DOI:
10.3791/51485-v

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11:27 min

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April 29, 2014

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, , , ,
1Genitourinary Section, Department of Medicine,Roswell Park Cancer Institute, 2Biological Sciences Platform,Sunnybrook Research Institute

Capitoli

  • 00:05Titolo
  • 02:13Orthotopic Kidney Tumor Implantation
  • 05:37Nephrectomy/Primary Tumor Removal
  • 06:51Monitoring Metastatic Progression and Localization at Endpoint
  • 09:47Results: Assessment of Metastasis Location and Severity
  • 10:19Conclusion

Summary

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Traduzione automatica

Models of spontaneous metastatic renal cell carcinoma (RCC) disease progression can be used for evaluating treatments in a clinically relevant setting. This protocol demonstrates different procedures for orthotopic kidney tumor cell implantation, proper nephrectomy, and finally outlines a necropsy guide for visual and bioluminescent scoring of metastatic burden and localization.

Tags

Metastatic Renal Cell CarcinomaMRCCMouse ModelNephrectomyOrthotopic Tumor ImplantationSyngeneic ModelBioluminescent ImagingMetastatic Disease ProgressionTherapeutic Testing

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