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Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
 

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Article DOI: 10.3791/51519-v • 08:27 min • July 24th, 2014
July 24th, 2014 •
1, 1, 2, 1
1NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health

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Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.

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Keywords: Human Pluripotent Stem Cells HPSCs Cell Culture Non-colony Type Monolayer NCM ROCK Inhibitor Extracellular Matrix Genetic Modification Transfection Transduction Regenerative Medicine Biopharmaceutical Applications
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