Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

Judith Konantz; Christopher L. Antos

doi:10.3791/51595

Rivista

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Biologia

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反向遗传吗啉使用方法心脏心室射出转染多难靶组织中的斑马鱼幼虫

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Biologia

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JoVE Journal Biologia

Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

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反向遗传吗啉使用方法心脏心室射出转染多难靶组织中的斑马鱼幼虫

DOI:

10.3791/51595-v

•

08:22 min

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June 17, 2014

•

Judith Konantz, Christopher L. Antos

¹DFG-Center for Regenerative Therapies Dresden,Technische Universität Dresden

Capitoli

00:05Titolo
01:20Preparation of Glass Needles and Morpholino Solution
02:26Larval Injections and Analysis
05:14Assessment of Regenerative Outgrowth
06:21Results: Transfection Using Cardiac Ventricular Injection
07:52Conclusion

Summary

Traduzione automatica

一个适应性强的反向遗传学方法，斑马鱼在后来的发展和生理平衡，如使用基因特异性吗啉脑室注射组织再生的阶段来评估基因的功能。

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