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Journal / Biology / High-throughput Image Analysis of Tumor…
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Biology

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JoVE Journal Biology
High-throughput Image Analysis of Tumor Spheroids
 

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

Article DOI: 10.3791/51639-v • 08:39 min • July 8th, 2014
July 8th, 2014 •
2,3, 1,3, 1,3, 3,4, 2,3, 1,3
1Raymond and Beverly Sackler Foundation, New Jersey, 2Histopathology and Imaging Shared Resource, Rutgers University, 3Rutgers Cancer Institute of New Jersey, Rutgers University, 4School of Natural Sciences, Institute for Advanced Study, New Jersey

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We present a high-throughput image analysis software application to measure the size of three-dimensional tumor spheroids imaged with bright-field microscopy. This application provides a fast and effective way to examine the effects of therapeutic drugs on spheroids, which is beneficial for researchers who wish to use spheroids in drug screens.

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Keywords: 3D Tumor Spheroids High-throughput Image Analysis Automated Measurement SpheroidSizer Software Active Contour Algorithm Graphical User Interface Drug Discovery In Vitro Model
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