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Analyse af epitelbeskadigelse Produceret af<em> Entamoeba histolytica</em> Infektion
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JoVE Journal Immunologia e infezioni
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Analyse af epitelbeskadigelse Produceret af<em> Entamoeba histolytica</em> Infektion

DOI:
10.3791/51668-v

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11:49 min

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June 12, 2014

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, , , , , ,
1Department of Infectomics and Molecular Pathogenesis,Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Molecular Biomedicine,Center for Research and Advanced Studies of the National Polytechnic Institute, 3Agency for Knowledge Commercialization,Center for Research and Advanced Studies of the National Polytechnic Institute

Capitoli

  • 00:05Titolo
  • 02:43Preparation of Cultures and Trophozoite Lysates
  • 04:00Preparation of Trophozoite Secreted Products
  • 04:58Interaction of MDCK Cells with Trophozoites, Trophozoite Lysates or Secreted Products
  • 07:33Measurement of Transepithelial Electrical Resistance (TER)
  • 08:42Results: Epithelial Damage by Entamoeba histolytica
  • 10:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

Human infektion med Entamoeba histolytica fører til amoebiasis, en væsentlig årsag til diarré i tropiske lande. Infektion initieres af patogen interaktioner med tarmepitelceller, provokerer åbningen af ​​celle-celle-kontakter, og dermed diarré, undertiden efterfulgt af lever-infektion. Denne artikel indeholder en model til at vurdere de tidlige vært-patogen interaktioner at forbedre vores forståelse af amoebiasis patogenese.

Tags

Entamoeba HistolyticaAmoebiasisEpithelial DamageTight JunctionsEhCPADH112Host-pathogen InteractionEpithelial Barrier DysfunctionTransepithelial Electrical ResistanceEpithelial Surface ReceptorsEpithelial Junctional MarkersParasite Localization

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