È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:34T. brucei Cell Culture
- 02:22Crude Mitochondria Isolation
- 03:56Editosome Purification
- 04:46RNA Preparation
- 06:13Fluorescence-based RNA Editing Assay
- 07:55Results: Determining the Assay Quality
- 08:54Conclusion
A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as therapeutics.
