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Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology
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JoVE Journal Medicina
Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

DOI:
10.3791/51807-v

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10:26 min

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August 18, 2014

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, , ,
1Department of Medicine, Renal division,University of Pittsburgh School of Medicine, 2Department of Pharmacology and Chemical Biology,University of Pittsburgh School of Medicine

Capitoli

  • 00:05Titolo
  • 01:34Tissue Preparation
  • 04:08Stimulation Protocols
  • 06:52Data Analysis
  • 07:42Results: Representative Bladder Smooth Muscle Tissue Contractility in Response to Various Stimuli
  • 09:45Conclusion

Summary

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This manuscript presents a simple, yet powerful, in vitro method for evaluating smooth muscle contractility in response to pharmacological agents or nerve stimulation. Main applications are drug screening and understanding tissue physiology, pharmacology, and pathology.

Tags

Bladder Smooth MuscleIn Vitro ContractilityLower Urinary Tract PharmacologyBladder FunctionMuscle StripIsometric Tension TransducerKrebs SolutionElectric Field StimulationNeurotransmissionSpinal Cord InjuryIntracellular PathwaysDrug Development

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