Rivista
/
/
איתור של אלטרנטיבי שחבור במהלך המעבר אפיתל- mesenchymal
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
DOI:

11:48 min

October 09, 2014

, ,

Capitoli

  • 00:05Titolo
  • 02:05EMT (Epithelial-mesenchymal Transition) Induction
  • 03:53Detection of Splice Isoforms Using qRT-PCR
  • 06:34Examination of Splice Isoforms at the Protein Level
  • 09:00Results: Alternative Splicing During Epithelial-mesenchymal Transition
  • 10:32Conclusion

Summary

Traduzione automatica

Alternative splicing regulation has been shown to contribute to the epithelial-mesenchymal transition (EMT), an essential cellular program in various physiological and pathological processes. Here we describe a method utilizing an inducible EMT model for the detection of alternative splicing during EMT.

Video correlati

Read Article