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Bioengineering

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JoVE Journal Bioengineering
Ordering Single Cells and Single Embryos in 3D Confinement
 

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Article DOI: 10.3791/51880-v • 14:22 min • September 18th, 2016
September 18th, 2016 •
1,2, 1,2, 1,2, 1,2
1Laboratory of Cell Physics, Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, 2Development and Stem Cells Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg

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We report a device and a new method to study cells and embryos. Single cells are precisely ordered in microcavity arrays. Their 3D confinement is a step towards 3D environments encountered in physiological conditions and allows organelle orientation. By controlling cell shape, this setup minimizes variability reported in standard assays.

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Keywords: 3D Confinement Single Cells Single Embryos High Content Screening Microcavities PDMS Egg Cups Extracellular Matrix Cell Phenotype 3D Environment Fission Yeast Cell Skeleton Drug Discovery Personalized Medicine C. Elegans Replica Molding
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