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非酶中，预浸润性乳腺癌病变的无血清组织培养的微球体的自然发生

JoVE Journal
Biologia

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JoVE Journal Biologia

Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

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非酶中，预浸润性乳腺癌病变的无血清组织培养的微球体的自然发生

DOI:

10.3791/51926-v

•

09:49 min

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November 08, 2014

•

Virginia Espina, Kirsten H. Edmiston, Lance A. Liotta

¹Center for Applied Proteomics and Molecular Medicine,George Mason University, ²Virginia Surgery Associates

Capitoli

00:05Titolo
02:03Tissue Acquisition and Grossing
04:22Maintenance of Established Organoid/Epithelial Cell Colonies
06:43Results: Representative Mammosphere Imaging
08:51Conclusion

Summary

Traduzione automatica

使用完整的组织组织体原代细胞培养提供了一个模型系统，模仿的多细胞在体内微环境。我们开发了一种无血清乳腺原发上皮细胞组织培养模式，延续了混合细胞培养系，并表现出分化的形态，没有酶的组织破坏。乳房组织体保持活力> 6个月。

Tags

Keywords: Non-enzymatic Serum-free Pre-invasive Breast Lesions Spontaneous Generation Mammospheres Breast Ductal Carcinoma In Situ (DCIS)Progenitor Cells Invasion Organoids Epithelial Cells Fibroblasts Cytogenetically Abnormal Invasive Progenitor Cells

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