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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
 

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Article DOI: 10.3791/52009-v • 08:14 min • October 28th, 2014
October 28th, 2014 •
1, 2, 3, 1, 1, 1
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Division of Hematology, The Children's Hospital of Philadelphia, 3Department of Pathology, The Children's Hospital of Philadelphia

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Here we describe a protocol for generating human induced pluripotent stem cells from peripheral blood using an episome based reprogramming strategy and histone deacetylase inhibitors.

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Efficient IPS Cell Generation Blood Episomes HDAC Inhibitors Integration-free Human Induced Pluripotent Stem Cells IPSCs Peripheral Blood Episomal Plasmids Histone Deacetylase Inhibitors Nonintegrating Reprogramming Vectors Cost Effective Method Vector Free IPSCs Single Transfection Small Molecules Epigenetic Reprogramming Peripheral Blood Mononuclear Cells PBMCs Erythrocyte Progenitor Cell Population Nontransmissible Episomal Plasmids OCT4 SOX2 KLF4 MYCL LIN28A P53 Short Hairpin RNA Nucleofection Enhanced Green Fluorescent Protein EGFP Identification Of Transfected Cells Replication-deficient Plasmid Epstein-Barr Nuclear Antigen 1 EBNA1
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