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Hohe Auflösung Elektronenmikroskopie der<em> Helicobacter pylori</em> Cag Typ IV-Sekretionssystem Pili Produziert in variierenden Bedingungen von Iron Verfügbarkeit
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
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Hohe Auflösung Elektronenmikroskopie der<em> Helicobacter pylori</em> Cag Typ IV-Sekretionssystem Pili Produziert in variierenden Bedingungen von Iron Verfügbarkeit

DOI:
10.3791/52122-v

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09:05 min

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November 21, 2014

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, ,
1Department of Medicine – Division of Infectious Diseases,Vanderbilt University School of Medicine, 2Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs

Capitoli

  • 00:05Titolo
  • 01:36H. pylori, Iron Availability and AGS Co-culture
  • 03:24SEM Sample Preparation
  • 05:01Imaging Parameters for High Resolution SEM Analyses
  • 05:46Quantifying H. pylori Cag-T4SS Pili
  • 07:07Results: Effect of Iron on H. pylori Cag-T4SS Pilus Biogenesis
  • 08:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

Tags

Helicobacter PyloriCag Type IV Secretion SystemPiliIron AvailabilityScanning Electron MicroscopyVirulence FactorGastric Cancer

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