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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
 

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Article DOI: 10.3791/52181-v • 11:00 min • January 12th, 2015
January 12th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University
* These authors contributed equally

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The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

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Keywords: Adipose-derived Stromal Cells ASCs Bone Marrow-derived Mesenchymal Stromal Cells BM-MSCs Osteogenesis Stromal Vascular Fraction SVF Tissue Engineering Critical-sized Calvarial Defects
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