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Developmental Biology

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JoVE Journal Developmental Biology
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
 

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

Article DOI: 10.3791/55120-v • 09:07 min • February 24th, 2017
February 24th, 2017 •
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1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine

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Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

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Keywords: Adipose-derived Stromal Cells BMPR-IB+ Calvarial Defect Craniofacial Regeneration In Vivo Assay Cell Isolation Cell Culture Cell Sorting Osteogenic Activity Regenerative Biology RBC Lysis Subcutaneous Fat
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