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Análisis de la especificidad de la proteína lisina metiltransferasas Usando SPOT Péptido Arrays
JoVE Journal
Biologia
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JoVE Journal Biologia
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
DOI:

08:48 min

November 29, 2014

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Capitoli

  • 00:05Titolo
  • 01:54Preparation of Peptide Arrays
  • 04:48Peptide Array Methylation
  • 06:18Results: Characterization of Methylated Peptide Arrays
  • 07:38Conclusion

Summary

Traduzione automatica

Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

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