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Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
 

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

Article DOI: 10.3791/52227-v • 11:31 min • November 30th, 2015
November 30th, 2015 •
1,2, 3, 1,4, 5, 6, 7, 6, 1,2
1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 3Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, 4Department of Psychology, University of Illinois at Urbana-Champaign, 5Department of Epidemiology, Mailman School of Public Health, Columbia University, 6Department of Epidemiology, University of Michigan School of Public Health, 7Center for Molecular Medicine and Genetics, Wayne State University School of Medicine

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We outline a methodology for the processing of whole blood to obtain a variety of components for further analysis. We have optimized a streamlined protocol that enables rapid, high-throughput simultaneous processing of whole blood samples in a non-clinical setting.

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Keywords: Whole Blood Fractionation PBMC Isolation DNA Extraction RNA Isolation Serum Separation Community-based Sampling Rapid Processing Population-based Studies
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