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Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
 

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

Article DOI: 10.3791/52239-v • 08:31 min • January 27th, 2015
January 27th, 2015 •
1, 2, 2, 1
1Department of Molecular Microbiology, Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, VIB, KU Leuven, 2Biomedical MRI Unit/ MoSAIC, Department of Imaging & Pathology, KU Leuven

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We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.

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Candida Albicans Biofilm Development Medically-relevant Foreign Bodies Mouse Subcutaneous Model Bioluminescence Imaging In Vivo Conditions Candida-infected Devices Central Venous Catheter Model System BALB/c Mice Three-dimensional Biofilm Architecture Quantification Of Fungal Biofilm Post Mortem Analysis Non-invasive Bioluminescence Imaging (BLI) Gaussia Princeps Luciferase Gene (gLuc) Cell Wall Attachment
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