Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Evaluation of Stem Cell Properties in Human…
JoVE Journal
Medicine

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicine
Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
 

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Article DOI: 10.3791/52259-v • 08:39 min • January 3rd, 2015
January 3rd, 2015 •
1,2, 1, 1,2
1Department of Biomedicine, University Hospital Basel, 2Department of Internal Medicine II, University Hospital Tübingen

Capitoli

Riepilogo
Automatic Translation

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

Tags

Stem Cell Properties Ovarian Carcinoma Cells Multi Cell-based Spheres Assays Single Cell-based Spheres Assays Cancer Stem Cells Tumor Initiation Maintenance Relapse Chemotherapies Ovarian CSCs Spheres Assay Non-adherent Culture Conditions Serum-free Medium Growth Factors Sphere Formation Protocols Side-by-side Analysis Multi Cell-based Assays Single Cell Plating Functional Assessment Accurate Results Density Of Plated Cells Titration Experiments Methylcellulose Supplementation Mechanical Artifacts
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter