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Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
 

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Article DOI: 10.3791/52259-v • 08:39 min • January 3rd, 2015
January 3rd, 2015 •
1,2, 1, 1,2
1Department of Biomedicine, University Hospital Basel, 2Department of Internal Medicine II, University Hospital Tübingen

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In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

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Keywords: Ovarian Carcinoma Cancer Stem Cells CSCs Spheres Assay Multi Cell-based Assays Single Cell-based Assays In Vitro Serum-free Medium Growth Factors Sphere Formation Methylcellulose
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