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Developmental Biology

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JoVE Journal Developmental Biology
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
 

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

Article DOI: 10.3791/52262-v • 09:18 min • February 4th, 2015
February 4th, 2015 •
1, 1, 1, 1, 1
1Ocular Trauma, U.S. Army Institute of Surgical Research

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The objective of this report is to describe the protocols to derive the retinal pigment epithelium (RPE) from induced pluripotent stem (iPS) cells using different sizes of embryoid bodies.

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Retinal Pigment Epithelium (RPE) Induced Pluripotent Stem (iPS) Cells Embryoid Bodies Stem Cell Differentiation Personalized Regenerative Medicine Tissue Engineering Microwell Plate Technology Immunocytochemistry Reverse-transcription Polymerase Chain Reaction (RT-PCR) Fluorescence-activated Cell Sorting (FACS) Analysis
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