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Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

DOI:
10.3791/56274-v

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10:48 min

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October 30, 2017

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1Center for Stem Cell Biology and Engineering,University of California, Santa Barbara

Capitoli

  • 00:05Titolo
  • 01:02Day 0: Day of Pluripotent Stem Cell Passage for Differentiation
  • 04:43Day of Enrichment to Passage 0 of Retinal Piment Epithelial Cells
  • 08:37Results: Representative Morphology and Confluence of Maturing RPE
  • 09:48Conclusion

Summary

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This protocol describes how to produce retinal pigment epithelial cells (RPE) from pluripotent stem cells. The method uses a combination of growth factors and small molecules to direct the differentiation of stem cells into immature RPE in fourteen days and mature, functional RPE after three months.

Tags

Retinal Pigment Epithelial CellsHuman Embryonic Stem CellsInduced Pluripotent Stem CellsRetinal DifferentiationDirected DifferentiationStem Cell BiologyEye ResearchRPERetinal DevelopmentStem Cell Disease ModelGrowth FactorsSmall MoleculesExtracellular MatrixHydrogelEDTACell PassagingCell RemovalUndifferentiated Stem CellsEfficiencyEnrichment

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