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JoVE Journal
Immunology and Infection

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Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
 

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Article DOI: 10.3791/52480-v 11:30 min January 7th, 2015
January 7th, 2015

Capitoli

Riepilogo

The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.

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Keywords: Endothelial Cells Leukocyte Recruitment Neutrophil Adhesion Mesenchymal Stem Cells In Vitro Vascular Model Flow-based Adhesion Assay TNF-alpha Inflammatory Regulation
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