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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
 

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Article DOI: 10.3791/52866-v • 07:51 min • May 21st, 2015
May 21st, 2015 •
1, 1, 1, 2, 1,3,4
1Department of Obstetrics & Gynecology, Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute, The University of Adelaide, 3Department of Immunology & Microbiology, Wayne State University School of Medicine, 4Perinatology Research Branch, NICHD/NIH/DHHS

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Automatic Translation

Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.

Tags

Isolation Leukocytes Murine Tissues Maternal-fetal Interface Immune Tolerance Pregnancy Coitus Fecundation Implantation Placenta Uterine Tissue Decidual Tissue Stromal Cells Infiltrating Leukocytes Neutrophils Macrophages Dendritic Cells Mast Cells T Cells B Cells NK Cells NKT Cells Micro-environment Pregnancy-related Complications Protocol
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