操作和在体外</em中>成熟非洲爪蟾卵母细胞，其次是胞浆内单精子注射，研究胚胎发育

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

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成熟非洲爪蟾卵母细胞，其次是胞浆内单精子注射，研究胚胎发育

DOI:

10.3791/52496-v

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09:22 min

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February 09, 2015

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Kei Miyamoto², David Simpson², John B. Gurdon²

¹Wellcome Trust/Cancer Research UK Gurdon Institute,University of Cambridge, ²Department of Zoology,University of Cambridge

Capitoli

00:05Titolo
01:14Preparations
03:47Injecting Oocytes with Oligos and Oocyte Maturation
05:55Intracytoplasmic Sperm Injection
08:12Results: ICS Embryo Survival
09:01Conclusion

Summary

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We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

操作和<em>在体外</em中>成熟<em>非洲爪蟾</em>卵母细胞，其次是胞浆内单精子注射，研究胚胎发育

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